ARG22988

anti-PLAP / Placental alkaline phosphatase antibody [H17E2]

anti-PLAP / Placental alkaline phosphatase antibody [H17E2] for IHC-Frozen sections,ELISA,Flow cytometry and Human

Overview

Product Description Mouse Monoclonal antibody [H17E2] recognizes PLAP / Placental alkaline phosphatase
Tested Reactivity Hu
Tested Application ELISA, FACS, IHC-Fr
Host Mouse
Clonality Monoclonal
Clone H17E2
Isotype IgG1
Target Name PLAP / Placental alkaline phosphatase
Antigen Species Human
Immunogen Placental membrane.
Conjugation Un-conjugated
Alternate Names PALP; PLAP; EC 3.1.3.1; Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1; PLAP-1; Alkaline phosphatase, placental type; ALP

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FACS10 µg/ml
IHC-FrAssay-dependent
Application Note IHC-Fr: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Arigo recommends the use of acetone fixation for frozen sections.
FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 250 Human ALPP

Swiss-port # P05187 Human Alkaline phosphatase, placental type

Gene Symbol ALPP
Gene Full Name alkaline phosphatase, placental
Background The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells. [provided by RefSeq, Jan 2015]
Calculated MW 58 kDa