ARG22988
anti-PLAP / Placental alkaline phosphatase antibody [H17E2]
anti-PLAP / Placental alkaline phosphatase antibody [H17E2] for IHC-Frozen sections,ELISA,Flow cytometry and Human
Overview
| Product Description | Mouse Monoclonal antibody [H17E2] recognizes PLAP / Placental alkaline phosphatase |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, FACS, IHC-Fr |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | H17E2 |
| Isotype | IgG1 |
| Target Name | PLAP / Placental alkaline phosphatase |
| Antigen Species | Human |
| Immunogen | Placental membrane. |
| Conjugation | Un-conjugated |
| Alternate Names | PALP; PLAP; EC 3.1.3.1; Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1; PLAP-1; Alkaline phosphatase, placental type; ALP |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | IHC-Fr: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Arigo recommends the use of acetone fixation for frozen sections. FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P05187 Human Alkaline phosphatase, placental type |
|---|---|
| Gene Symbol | ALPP |
| Gene Full Name | alkaline phosphatase, placental |
| Background | The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells. [provided by RefSeq, Jan 2015] |
| Calculated MW | 58 kDa |
