ARG55680

anti-PLOD1 antibody

anti-PLOD1 antibody for Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes PLOD1
Tested Reactivity Hu
Predict Reactivity Ms
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PLOD1
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 66-94 (N-terminus) of Human PLOD1.
Conjugation Un-conjugated
Alternate Names EC 1.14.11.4; EDS6; Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1; Lysyl hydroxylase 1; LH; PLOD; LLH; LH1

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control A431

Properties

Form Liquid
Purification Purification with Protein A and immunogen peptide.
Buffer PBS and 0.09% (W/V) Sodium azide.
Preservative 0.09% (W/V) Sodium azide.
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5351 Human PLOD1

Swiss-port # Q02809 Human Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1

Gene Symbol PLOD1
Gene Full Name procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1
Background Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity. [provided by RefSeq, Jul 2008]
Function Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. [UniProt]
Cellular Localization Rough endoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side
Calculated MW 84 kDa