ARG66868
anti-PNPLA3 / Adiponutrin antibody
anti-PNPLA3 / Adiponutrin antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PNPLA3 / Adiponutrin |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PNPLA3 / Adiponutrin |
| Antigen Species | Human |
| Immunogen | Recombinant full-length protein of Human PNPLA3 / Adiponutrin. |
| Conjugation | Un-conjugated |
| Alternate Names | ADPN; Calcium-independent phospholipase A2-epsilon; iPLA(2)epsilon; Adiponutrin; Patatin-like phospholipase domain-containing protein 3; iPLA2-epsilon; EC 2.3.1.-; C22orf20; EC 3.1.1.3; Acylglycerol O-acyltransferase |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 110 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 30% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q91WW7 Mouse Patatin-like phospholipase domain-containing protein 3 Swiss-port # Q9NST1 Human Patatin-like phospholipase domain-containing protein 3 |
|---|---|
| Gene Symbol | PNPLA3 |
| Gene Full Name | patatin-like phospholipase domain containing 3 |
| Background | The protein encoded by this gene is a triacylglycerol lipase that mediates triacylglycerol hydrolysis in adipocytes. The encoded protein, which appears to be membrane bound, may be involved in the balance of energy usage/storage in adipocytes. [provided by RefSeq, Jul 2008] |
| Function | Specifically catalyzes coenzyme A (CoA)-dependent acylation of 1-acyl-sn-glycerol 3-phosphate (2-lysophosphatidic acid/LPA) to generate phosphatidic acid (PA), an important metabolic intermediate and precursor for both triglycerides and glycerophospholipids. Does not esterify other lysophospholipids. Acyl donors are long chain (at least C16) fatty acyl-CoAs: arachidonoyl-CoA, linoleoyl-CoA, oleoyl-CoA and at a lesser extent palmitoyl-CoA (PubMed:22560221). Additionally possesses low triacylglycerol lipase and CoA-independent acylglycerol transacylase activities and thus may play a role in acyl-chain remodeling of triglycerides (PubMed:15364929, PubMed:20034933, PubMed:22560221). [UniProt] |
| Cellular Localization | Membrane; Single-pass type II membrane protein. [UniProt] |
| Calculated MW | 53 kDa |
