ARG63382
anti-POLL / POLkappa antibody
anti-POLL / POLkappa antibody for Western blot and Human
Gene Regulation antibody
Overview
Product Description | Goat Polyclonal antibody recognizes POLL / POLkappa |
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Tested Reactivity | Hu |
Tested Application | WB |
Specificity | It has been brought to our attention that this product may crossreact with Pol beta (NP_002681), an evolutionarilly related polymerase with a predicted size of 39 kDa. The overall % of identity is not very high (about 32% in the core) but considering only the peptide that we used as immunogen the two share the sequence YREP**R** at the C-terminus. Further the protein structures appear to be very similar in this region. |
Host | Goat |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | POLL / POLkappa |
Antigen Species | Human |
Immunogen | C-LGLPYREPAERDW |
Conjugation | Un-conjugated |
Alternate Names | DNA polymerase beta-2; BETAN; EC 4.2.99.-; EC 2.7.7.7; Pol beta2; Pol Lambda; POLKAPPA; DNA polymerase kappa; DNA polymerase lambda |
Application Instructions
Application Suggestion |
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Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Form | Liquid |
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Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
Preservative | 0.02% Sodium azide |
Stabilizer | 0.5% BSA |
Concentration | 0.5 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Background | This gene encodes a DNA polymerase. DNA polymerases catalyze DNA-template-directed extension of the 3'-end of a DNA strand. This particular polymerase, which is a member of the X family of DNA polymerases, likely plays a role in non-homologous end joining and other DNA repair processes. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2010] |
Research Area | Gene Regulation antibody |
Calculated MW | 63 kDa |