ARG63382

anti-POLL / POLkappa antibody

anti-POLL / POLkappa antibody for Western blot and Human

Gene Regulation antibody

Overview

Product Description Goat Polyclonal antibody recognizes POLL / POLkappa
Tested Reactivity Hu
Tested Application WB
Specificity It has been brought to our attention that this product may crossreact with Pol beta (NP_002681), an evolutionarilly related polymerase with a predicted size of 39 kDa. The overall % of identity is not very high (about 32% in the core) but considering only the peptide that we used as immunogen the two share the sequence YREP**R** at the C-terminus. Further the protein structures appear to be very similar in this region.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name POLL / POLkappa
Antigen Species Human
Immunogen C-LGLPYREPAERDW
Conjugation Un-conjugated
Alternate Names DNA polymerase beta-2; BETAN; EC 4.2.99.-; EC 2.7.7.7; Pol beta2; Pol Lambda; POLKAPPA; DNA polymerase kappa; DNA polymerase lambda

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.5 - 1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 27343 Human POLL

Swiss-port # Q9UGP5 Human DNA polymerase lambda

Background This gene encodes a DNA polymerase. DNA polymerases catalyze DNA-template-directed extension of the 3'-end of a DNA strand. This particular polymerase, which is a member of the X family of DNA polymerases, likely plays a role in non-homologous end joining and other DNA repair processes. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2010]
Research Area Gene Regulation antibody
Calculated MW 63 kDa