ARG57646

anti-POLR2B antibody

anti-POLR2B antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes POLR2B
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name POLR2B
Antigen Species Human
Immunogen Recombinant protein of Human POLR2B.
Conjugation Un-conjugated
Alternate Names RNA polymerase II subunit 2; RPB2; POL2RB; DNA-directed RNA polymerase II subunit B; DNA-directed RNA polymerase II 140 kDa polypeptide; RNA polymerase II subunit B2; DNA-directed RNA polymerase II subunit RPB2; hRPB140; EC 2.7.7.6

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HepG2
Observed Size ~ 134 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 231329 Mouse POLR2B

GeneID: 5431 Human POLR2B

Swiss-port # P30876 Human DNA-directed RNA polymerase II subunit RPB2

Swiss-port # Q8CFI7 Mouse DNA-directed RNA polymerase II subunit RPB2

Gene Symbol POLR2B
Gene Full Name polymerase (RNA) II (DNA directed) polypeptide B, 140kDa
Background This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
Function DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity). [UniProt]
Cellular Localization Nucleus [UniProt]
Calculated MW 134 kDa