ARG66454

anti-POLR2I / RPB8 antibody

anti-POLR2I / RPB8 antibody for Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes POLR2I / RPB8
Tested Reactivity Hu, Ms, Rat
Predict Reactivity Bov, Chk, Dog, Mk, Pig, Rb
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name POLR2I / RPB8
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide within the center region of Human POLR2I / RPB8.
Conjugation Un-conjugated
Alternate Names RPB9; RPB14.5; hRPB14.5; DNA-directed RNA polymerase II subunit I; DNA-directed RNA polymerase II subunit RPB9; RNA polymerase II subunit B9; RNA polymerase II 14.5 kDa subunit

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol.
Preservative 0.01% Sodium azide
Stabilizer 30% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5438 Human POLR2I

GeneID: 69920 Mouse POLR2I

Swiss-port # P36954 Human DNA-directed RNA polymerase II subunit RPB9

Swiss-port # P60898 Mouse DNA-directed RNA polymerase II subunit RPB9

Gene Symbol POLR2I
Gene Full Name polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa
Background This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity. [provided by RefSeq, Jul 2008]
Function DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB9 is part of the upper jaw surrounding the central large cleft and thought to grab the incoming DNA template (By similarity). [UniProt]
Cellular Localization Nucleus, nucleolus. [UniProt]
Calculated MW 15 kDa