ARG57029
anti-PPM1F antibody [6D11]
anti-PPM1F antibody [6D11] for ICC/IF,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [6D11] recognizes PPM1F |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 6D11 |
| Isotype | IgG1, kappa |
| Target Name | PPM1F |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-454 of Human PPM1F. |
| Conjugation | Un-conjugated |
| Alternate Names | hFEM-2; FEM-2; CAMKP; POPX2; EC 3.1.3.16; Partner of PIX 2; hFem-2; Protein phosphatase 1F; CaM-kinase phosphatase; CaMKPase; Ca; Protein fem-2 homolog; 2+ |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PPM1F |
| Gene Full Name | protein phosphatase, Mg2+/Mn2+ dependent, 1F |
| Background | The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined. [provided by RefSeq, Jul 2008] |
| Function | Dephosphorylates and concomitantly deactivates CaM-kinase II activated upon autophosphorylation, and CaM-kinases IV and I activated upon phosphorylation by CaM-kinase kinase. Promotes apoptosis. [UniProt] |
| Calculated MW | 50 kDa |
