ARG57085
anti-PPME1 antibody [29G3]
anti-PPME1 antibody [29G3] for Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [29G3] recognizes PPME1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 29G3 |
| Isotype | IgG1, kappa |
| Target Name | PPME1 |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-386 of Human PPME1. |
| Conjugation | Un-conjugated |
| Alternate Names | PME-1; EC 3.1.1.89; Protein phosphatase methylesterase 1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9Y570 Human Protein phosphatase methylesterase 1 |
|---|---|
| Gene Symbol | PPME1 |
| Gene Full Name | protein phosphatase methylesterase 1 |
| Background | This gene encodes a protein phosphatase methylesterase localized to the nucleus. The encoded protein acts on the protein phosphatase-2A catalytic subunit and supports the ERK pathway through dephosphorylation of regulatory proteins. It plays a role in malignant glioma progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012] |
| Function | Demethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro) and PPP2CA. Binding to PPP2CA displaces the manganese ion and inactivates the enzyme. [UniProt] |
| Calculated MW | 42 kDa |
| PTM | Phosphorylated by SIK1 following increases in intracellular sodium, leading to dissociation from the protein phosphatase 2A (PP2A) complex and subsequent dephosphorylation of sodium/potassium-transporting ATPase ATP1A1. |
