ARG63153

anti-PPP1R15A / GADD34 antibody

anti-PPP1R15A / GADD34 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cell Biology and Cellular Response antibody; Gene Regulation antibody

Overview

Product Description Goat Polyclonal antibody recognizes PPP1R15A / GADD34
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name PPP1R15A / GADD34
Antigen Species Human
Immunogen C-AAALDLSGRRG
Conjugation Un-conjugated
Alternate Names Growth arrest and DNA damage-inducible protein GADD34; Protein phosphatase 1 regulatory subunit 15A; Myeloid differentiation primary response protein MyD116 homolog; GADD34

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µg/ml
ICC/IF10 µg/ml
IHC-P2 - 4 µg/ml
WB0.3 - 1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 23645 Human PPP1R15A

Swiss-port # O75807 Human Protein phosphatase 1 regulatory subunit 15A

Background This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation. [provided by RefSeq, Jul 2008]
Research Area Cell Biology and Cellular Response antibody; Gene Regulation antibody
Calculated MW 73 kDa
PTM Phosphorylated at multiple Ser/Thr residues. Phosphorylated on tyrosine by LYN; which impairs its antiproliferative activity. Phosphorylation at Tyr-262 enhances proteasomal degradation, this position is dephosphorylated by PTPN2.
Polyubiquitinated. Exhibits a rapid proteasomal degradation with a half-life under 1 hour, ubiquitination depends on endoplasmic reticulum association.