ARG63153
anti-PPP1R15A / GADD34 antibody
anti-PPP1R15A / GADD34 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody; Gene Regulation antibody
Overview
Product Description | Goat Polyclonal antibody recognizes PPP1R15A / GADD34 |
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Tested Reactivity | Hu |
Tested Application | FACS, ICC/IF, IHC-P, WB |
Host | Goat |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | PPP1R15A / GADD34 |
Antigen Species | Human |
Immunogen | C-AAALDLSGRRG |
Conjugation | Un-conjugated |
Alternate Names | Growth arrest and DNA damage-inducible protein GADD34; Protein phosphatase 1 regulatory subunit 15A; Myeloid differentiation primary response protein MyD116 homolog; GADD34 |
Application Instructions
Application Suggestion |
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Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Form | Liquid |
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Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
Preservative | 0.02% Sodium azide |
Stabilizer | 0.5% BSA |
Concentration | 0.5 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links |
Swiss-port # O75807 Human Protein phosphatase 1 regulatory subunit 15A |
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Background | This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation. [provided by RefSeq, Jul 2008] |
Research Area | Cell Biology and Cellular Response antibody; Gene Regulation antibody |
Calculated MW | 73 kDa |
PTM | Phosphorylated at multiple Ser/Thr residues. Phosphorylated on tyrosine by LYN; which impairs its antiproliferative activity. Phosphorylation at Tyr-262 enhances proteasomal degradation, this position is dephosphorylated by PTPN2. Polyubiquitinated. Exhibits a rapid proteasomal degradation with a half-life under 1 hour, ubiquitination depends on endoplasmic reticulum association. |