ARG64935

anti-PPP4C antibody

anti-PPP4C antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat

Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes PPP4C
Tested Reactivity Hu, Rat
Predict Reactivity Dog
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name PPP4C
Antigen Species Human
Immunogen C-PQETRGIPSKKP
Conjugation Un-conjugated
Alternate Names PPP4; PPX; PP-X; Pp4; PPH3; EC 3.1.3.16; Serine/threonine-protein phosphatase 4 catalytic subunit; PP4; PP4C; Protein phosphatase X

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P2 - 4 µg/ml
WB0.1 - 0.3 µg/ml
Application Note IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by antigen affinity chromatography.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 171366 Rat PPP4C

GeneID: 5531 Human PPP4C

Swiss-port # P60510 Human Serine/threonine-protein phosphatase 4 catalytic subunit

Swiss-port # Q5BJ92 Rat Serine/threonine-protein phosphatase 4 catalytic subunit

Gene Symbol PPP4C
Gene Full Name protein phosphatase 4, catalytic subunit
Function Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity). In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin. [UniProt]
Research Area Signaling Transduction antibody
Calculated MW 35 kDa
PTM Methylation at the C-terminal Leu-307 is critical for interactions with regulatory subunits and functions in DNA repair.