ARG40092
anti-PRAME antibody
anti-PRAME antibody for Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PRAME |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PRAME |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-200 of Human PRAME (NP_006106.1). |
| Conjugation | Un-conjugated |
| Alternate Names | OIP4; MAPE; Melanoma antigen preferentially expressed in tumors; CT130; OIP-4; Preferentially expressed antigen of melanoma; Opa-interacting protein 4 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | Rat lung, Mouse liver and U2OS | ||||
| Observed Size | 58 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P78395 Human Melanoma antigen preferentially expressed in tumors |
|---|---|
| Gene Symbol | PRAME |
| Gene Full Name | preferentially expressed antigen in melanoma |
| Background | This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014] |
| Function | Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis. [UniProt] |
| Cellular Localization | Nucleus. Cell membrane. [UniProt] |
| Calculated MW | 58 kDa |
