ARG59011
anti-PRKAR1A / PKA RI alpha antibody
anti-PRKAR1A / PKA RI alpha antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PRKAR1A / PKA RI alpha |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PRKAR1A / PKA RI alpha |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to E2-E81 of Human PRKAR1A. |
| Conjugation | Un-conjugated |
| Alternate Names | Tissue-specific extinguisher 1; ADOHR; CAR; PRKAR1; ACRDYS1; TSE1; cAMP-dependent protein kinase type I-alpha regulatory subunit; CNC; CNC1; PPNAD1; PKR1; PKA 1 alpha |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: By heat mediation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P09456 Rat cAMP-dependent protein kinase type I-alpha regulatory subunit Swiss-port # P10644 Human cAMP-dependent protein kinase type I-alpha regulatory subunit |
|---|---|
| Gene Symbol | PRKAR1A |
| Gene Full Name | protein kinase, cAMP-dependent, regulatory, type I, alpha |
| Background | cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. This gene encodes one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Several alternatively spliced transcript variants encoding two different isoforms have been observed. [provided by RefSeq, Jan 2013] |
| Function | Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. [UniProt] |
| Cellular Localization | Cell membrane. [UniProt] |
| Calculated MW | 43 kDa |
| PTM | The pseudophosphorylation site binds to the substrate-binding region of the catalytic chain, resulting in the inhibition of its activity. [UniProt] |
