ARG54089
anti-PRMT1 antibody
anti-PRMT1 antibody for Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody
Overview
| Product Description | Mouse Monoclonal antibody recognizes PRMT1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Target Name | PRMT1 |
| Antigen Species | Human |
| Immunogen | Purified recombinant human PRMT1 protein fragments expressed in E.coli. |
| Conjugation | Un-conjugated |
| Alternate Names | IR1B4; EC 2.1.1.125; ANM1; Protein arginine N-methyltransferase 1; Histone-arginine N-methyltransferase PRMT1; HCP1; EC 2.1.1.-; Interferon receptor 1-bound protein 4; HRMT1L2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Observed Size | 42 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q99873 Human Protein arginine N-methyltransferase 1 |
|---|---|
| Gene Symbol | PRMT1 |
| Gene Full Name | protein arginine methyltransferase 1 |
| Background | Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1,histone H2,H3 and H4,PIAS1,HNRNPA1,HNRNPD,NFATC2IP,SUPT5H,TAF15 and EWS.Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a,respectively),a specific tag for epigenetic transcriptional activation.Together with dimethylated PIAS1,represses STAT1 transcriptional activity,in the late phase of interferon gamma (IFN-gamma) signaling.May be involved in the regulation of TAF15 transcriptional activity,act as an activator of estrogen receptor (ER)-mediated transactivation,play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation,by modulating p38 MAPK pathway.Methylates FOXO1 and retains it in the nucleus increasing its transcriptional activity. |
| Function | Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates FOXO1 and retains it in the nucleus increasing its transcriptional activity. [UniProt] |
| Cellular Localization | Nucleus. Nucleus > nucleoplasm by similarity. Cytoplasm > cytosol by similarity. Note: Mostly found in the cytoplasm. Colocalizes with CHTOP within the nucleus. Low levels detected also in the chromatin fraction by similarity. |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody |
| Calculated MW | 42 kDa |
