ARG59786
anti-PRPF3 antibody
anti-PRPF3 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PRPF3 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PRPF3 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-280 of Human PRPF3 (NP_004689.1). |
| Conjugation | Un-conjugated |
| Alternate Names | Pre-mRNA-splicing factor 3; Prp3p; SNRNP90; U4/U6 small nuclear ribonucleoprotein Prp3; PRP3; hPrp3; U4/U6 snRNP 90 kDa protein; HPRP3; HPRP3P; RP18 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Mouse testis and BT-474 | ||||||||
| Observed Size | 100 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O43395 Human U4/U6 small nuclear ribonucleoprotein Prp3 Swiss-port # Q922U1 Mouse U4/U6 small nuclear ribonucleoprotein Prp3 |
|---|---|
| Gene Symbol | PRPF3 |
| Gene Full Name | pre-mRNA processing factor 3 |
| Background | The removal of introns from nuclear pre-mRNAs occurs on complexes called spliceosomes, which are made up of 4 small nuclear ribonucleoprotein (snRNP) particles and an undefined number of transiently associated splicing factors. This gene product is one of several proteins that associate with U4 and U6 snRNPs. Mutations in this gene are associated with retinitis pigmentosa-18. [provided by RefSeq, Jul 2008] |
| Function | Participates in pre-mRNA splicing. Part of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome. [UniProt] |
| Cellular Localization | Nucleus speckle. Note=Colocalizes with spliceosomal snRNPs. [UniProt] |
| Highlight | Related products: PRPF3 antibodies; Anti-Rabbit IgG secondary antibodies; Related news: Gene therapy for retinitis pigmentosa (RP) |
| Calculated MW | 78 kDa |
| PTM | Ubiquitinated. Undergoes 'Lys-63'-linked polyubiquitination by PRPF19 and deubiquitination by USP4. 'Lys-63'-linked ubiquitination increases the affinity for PRPF8 and may regulate the assembly of the U4/U6-U5 tri-snRNP complex. [UniProt] |
