ARG57463

anti-PSMB8 / LMP7 antibody

anti-PSMB8 / LMP7 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat

Overview

Product Description Mouse Monoclonal antibody recognizes PSMB8 / LMP7
Tested Reactivity Hu, Rat
Tested Application IHC-P, WB
Host Mouse
Clonality Monoclonal
Isotype IgG1
Target Name PSMB8 / LMP7
Antigen Species Human
Immunogen Purified recombinant fragment of Human PSMB8 expressed in E. coli.
Conjugation Un-conjugated
Alternate Names Proteasome subunit beta-5i; Multicatalytic endopeptidase complex subunit C13; Proteasome subunit beta type-8; D6S216E; Really interesting new gene 10 protein; JMP; LMP7; NKJO; PSMB5i; Proteasome component C13; EC 3.4.25.1; RING10; Low molecular mass protein 7; ALDD; D6S216; Macropain subunit C13

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:200 - 1:1000
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Ascitic fluid
Buffer Ascitic fluid and 0.03% Sodium azide.
Preservative 0.03% Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 24968 Rat PSMB8

GeneID: 5696 Human PSMB8

Swiss-port # P28062 Human Proteasome subunit beta type-8

Swiss-port # P28064 Rat Proteasome subunit beta type-8

Gene Symbol PSMB8
Gene Full Name proteasome subunit beta 8
Background The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit. [provided by RefSeq, Jul 2008]
Function The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Acts as a major component of interferon gamma-induced sensitivity. Plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. May be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency. Required for the differentiation of preadipocytes into adipocytes. [UniProt]
Cellular Localization Nucleus.
Calculated MW 30 kDa
PTM Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.