ARG64947

anti-PSMB8 / LMP7 antibody

anti-PSMB8 / LMP7 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cell Biology and Cellular Response antibody

Overview

Product Description Goat Polyclonal antibody recognizes PSMB8 / LMP7
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Cow, Dog, Pig
Tested Application IHC-P, WB
Specificity This antibody is expected to recognize both reported isoforms (NP_004150.1; NP_683720.2).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name PSMB8 / LMP7
Antigen Species Human
Immunogen C-DVSDLLHQYREANQ
Conjugation Un-conjugated
Alternate Names Proteasome subunit beta-5i; Multicatalytic endopeptidase complex subunit C13; Proteasome subunit beta type-8; D6S216E; Really interesting new gene 10 protein; JMP; LMP7; NKJO; PSMB5i; Proteasome component C13; EC 3.4.25.1; RING10; Low molecular mass protein 7; ALDD; D6S216; Macropain subunit C13

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P3 - 5 µg/ml
WB0.01 - 0.03 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5696 Human PSMB8

Swiss-port # P28062 Human Proteasome subunit beta type-8

Background The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit. [provided by RefSeq, Jul 2008]
Research Area Cell Biology and Cellular Response antibody
Calculated MW 30 kDa
PTM Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.