ARG43998
anti-PSMD4 antibody
anti-PSMD4 antibody for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PSMD4 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ELISA, FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PSMD4 |
| Antigen Species | Human |
| Immunogen | Human PSMD4 recombinant protein |
| Expression System | E.coli |
| Conjugation | Un-conjugated |
| Protein Full Name | 26S proteasome non-ATPase regulatory subunit 4 |
| Alternate Names | PSMD4; Proteasome 26S Subunit Ubiquitin Receptor, Non-ATPase 4; AF; Rpn10; AF-1; S5A; Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 4; 26S Proteasome Non-ATPase Regulatory Subunit 4; 26S Proteasome Regulatory Subunit S5A; Proteasome 26S Subunit, Non-ATPase 4; Multiubiquitin Chain-Binding Protein; Antisecretory Factor 1; MCB1; ASF; 26S Proteasome Regulatory Subunit RPN10; S5a/Antisecretory Factor Protein; Angiocidin; PUB-R5 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified with Immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O35226 Mouse 26S proteasome non-ATPase regulatory subunit 4 Swiss-port # P55036 Human 26S proteasome non-ATPase regulatory subunit 4 |
|---|---|
| Gene Symbol | PSMD4 |
| Gene Full Name | Proteasome 26S Subunit Ubiquitin Receptor, Non-ATPase 4 |
| Background | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. Pseudogenes have been identified on chromosomes 10 and 21. |
| Function | Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMD4 acts as an ubiquitin receptor subunit through ubiquitin-interacting motifs and selects ubiquitin-conjugates for destruction. Displays a preferred selectivity for longer polyubiquitin chains. |
| Cellular Localization | Proteasome |
| Calculated MW | 41 kDa |
| PTM | Isopeptide bond, Phosphoprotein, Ubl conjugation |
