ARG55484

anti-PSME1 antibody

anti-PSME1 antibody for ICC/IF,Western blot and Human,Mouse

Cell Biology and Cellular Response antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes PSME1
Tested Reactivity Hu, Ms
Predict Reactivity Rat
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PSME1
Antigen Species Human
Immunogen Recombinant protein of Human PSME1
Conjugation Un-conjugated
Alternate Names REG-alpha; Proteasome activator 28 subunit alpha; PA28alpha; Activator of multicatalytic protease subunit 1; 11S regulator complex subunit alpha; Interferon gamma up-regulated I-5111 protein; PA28A; IGUP I-5111; IFI5111; Proteasome activator complex subunit 1; REGalpha; PA28a

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse heart and BT474
Observed Size ~ 30 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 19186 Mouse PSME1

GeneID: 5720 Human PSME1

Swiss-port # P97371 Mouse Proteasome activator complex subunit 1

Swiss-port # Q06323 Human Proteasome activator complex subunit 1

Gene Symbol PSME1
Gene Full Name proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)
Background The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Function Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome. [UniProt]
Research Area Cell Biology and Cellular Response antibody
Calculated MW 29 kDa