ARG55445

anti-PSME2 antibody

anti-PSME2 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,Flow cytometry and Human,Mouse

Cell Biology and Cellular Response antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes PSME2
Tested Reactivity Hu, Ms
Predict Reactivity Bov, Rat
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PSME2
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 210-239 (C-terminus) of Human PSME2.
Conjugation Un-conjugated
Alternate Names 11S regulator complex subunit beta; REG-beta; PA28beta; Proteasome activator 28 subunit beta; Activator of multicatalytic protease subunit 2; PA28b; PA28B; Proteasome activator complex subunit 2; REGbeta

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:50
IHC-P1:50 - 1:100
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HepG2

Properties

Form Liquid
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 19188 Mouse PSME2

GeneID: 5721 Human PSME2

Swiss-port # P97372 Mouse Proteasome activator complex subunit 2

Swiss-port # Q9UL46 Human Proteasome activator complex subunit 2

Gene Symbol PSME2
Gene Full Name proteasome (prosome, macropain) activator subunit 2 (PA28 beta)
Background The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the beta subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three beta and three alpha subunits combine to form a heterohexameric ring. Six pseudogenes have been identified on chromosomes 4, 5, 8, 10 and 13. [provided by RefSeq, Jul 2008]
Function Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome. [UniProt]
Research Area Cell Biology and Cellular Response antibody
Calculated MW 27 kDa