ARG52405
anti-PTPN3 / PTPH1 phospho (Ser459) antibody
anti-PTPN3 / PTPH1 phospho (Ser459) antibody for Western blot and Human,Mouse
Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PTPN3 / PTPH1 phospho (Ser459) |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Bov, Dog, NHuPrm, Sheep |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PTPN3 / PTPH1 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic phospho-peptide around Ser459. |
| Conjugation | Un-conjugated |
| Alternate Names | PTPH1; EC 3.1.3.48; Protein-tyrosine phosphatase H1; Tyrosine-protein phosphatase non-receptor type 3; PTP-H1 |
Application Instructions
| Application Suggestion |
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| Application Note | Specific for ~104k PTPH1 protein phosphorylated at Ser459 . Immunolabeling is blocked by preadsorption of the antibody with the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is also completely eliminated by treatment with λ phosphatase. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # A2ALK8 Mouse Tyrosine-protein phosphatase non-receptor type 3 Swiss-port # P26045 Human Tyrosine-protein phosphatase non-receptor type 3 |
|---|---|
| Gene Symbol | PTPH1 |
| Gene Full Name | protein tyrosine phosphatase, non-receptor type 3 |
| Background | Protein-tyrosine Phosphatase H1 (PTPH1) has recently been identified as a specific p38γ MAPK phosphatase which is mediated through PDZ interaction (Hou et al., 2010). Ras has been demonstrated to increase both p38γ and PTPH1 protein expression, and there is a coupling of increased p38γ and PTPH1 protein expression in primary colon cancer tissues (Hou et al., 2010). Phosphorylation of PTPH1 at Ser459 leads to PTPH1 stabilization, which plays an important role in Ras oncogenesis and stress response (Hou et al., 2012). Additionally, phosphorylation of PTPH1 Ser459 reveals a novel mechanism by which MAPK signals through PTPH1 to regulate cellular response (Hou et al., 2012). |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 104 kDa |
