ARG64671
anti-PU.1 antibody
anti-PU.1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Gene Regulation antibody; Immune System antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes PU.1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Rat |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognise both reported isoforms (NP_001074016.1; NP_003111.2). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PU.1 |
| Antigen Species | Human |
| Immunogen | C-DLYQRQTHEYY |
| Conjugation | Un-conjugated |
| Alternate Names | Transcription factor PU.1; SPI-1; OF; SPI-A; PU.1; SFPI1; 31 kDa-transforming protein |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Research Area | Gene Regulation antibody; Immune System antibody |
| Calculated MW | 31 kDa |
