ARG54440
anti-PUMA antibody
anti-PUMA antibody for Western blot,Immunohistochemistry and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody
Overview
Product Description | Rabbit Polyclonal antibody recognizes PUMA |
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Tested Reactivity | Hu |
Tested Application | IHC, WB |
Specificity | This antibody recognizes human and mouse PUMA (23kDa). |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | PUMA |
Antigen Species | Human |
Immunogen | Synthetic peptide corresponding to aa 2-16 of human PUMA-alpha (accession no. NP_055232). This sequence is identical in mouse PUMA. |
Conjugation | Un-conjugated |
Alternate Names | PUMA; p53 up-regulated modulator of apoptosis; JFY-1; JFY1; Bcl-2-binding component 3 |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
Positive Control | K562 and Human breast carcinoma |
Properties
Form | Liquid |
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Purification | Immunoaffinity chroma-tography |
Buffer | PBS (pH 7.4) and 0.02% Sodium azide |
Preservative | 0.02% Sodium azide |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Gene Symbol | BBC3 |
Gene Full Name | BCL2 binding component 3 |
Background | PUMA (p53 Upregulated Modulator of Apoptosis) is a pro-apoptotic Bcl-2 family member that encodes two BH3 domain-containing proteins, PUMA- and PUMA- . PUMA proteins bind Bcl-2, localize to mitochondria, and induce cytochrome c release and apoptosis in response to p53. |
Function | Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Isoform 3 fails to show any growth-inhibitory or apoptotic activity. [UniProt] |
Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody |
Calculated MW | 26 kDa |