ARG57486

anti-PUMA antibody

anti-PUMA antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes PUMA
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-P, WB
Specificity This antibody detects endogenous levels of total PUMA.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PUMA
Antigen Species Human
Immunogen Synthetic peptide derived from Human PUMA.
Conjugation Un-conjugated
Alternate Names PUMA; p53 up-regulated modulator of apoptosis; JFY-1; JFY1; Bcl-2-binding component 3

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:50
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified by affinity chromatography.
Buffer PBS (pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 170770 Mouse BBC3

GeneID: 27113 Human BBC3

GeneID: 317673 Rat BBC3

Gene Symbol BBC3
Gene Full Name BCL2 binding component 3
Background This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]
Function Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Isoform 3 fails to show any growth-inhibitory or apoptotic activity. [UniProt]
Calculated MW 26 kDa