ARG52390
anti-Parkin phospho (Ser378) antibody
anti-Parkin phospho (Ser378) antibody for Western blot and Human
Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Parkin phospho (Ser378) |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Parkin |
| Antigen Species | Human |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser378 conjugated to KLH |
| Conjugation | Un-conjugated |
| Alternate Names | AR-JP; EC 6.3.2.-; PRKN; Parkinson juvenile disease protein 2; E3 ubiquitin-protein ligase parkin; PDJ; Parkin; LPRS2; Parkinson disease protein 2 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | Specific for the ~52k parkin protein phosphorylated at Ser378. Immunolabeling of the parkin band is absent in parkin S378 mutants. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O60260 Human E3 ubiquitin-protein ligase parkin |
|---|---|
| Gene Symbol | PARK2 |
| Gene Full Name | parkin RBR E3 ubiquitin protein ligase |
| Background | Parkin is an E3 ligase in the ubiquitin-proteasome system. Hereditary Parkinson’s disease is most commonly caused by mutations in the parkin gene and is characterized by the progressive loss of dopaminergic neurons and the presence of Lewy bodies in the substania nigra (Jenner et al.,1992). Recent evidence suggests that phosphorylation of parkin at Ser378 may have an important regulatory role on its E3 ubiquitin ligase activity (Yamamoto et al., 2005). |
| Research Area | Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody |
| Calculated MW | 52 kDa |
| PTM | Auto-ubiquitinates in an E2-dependent manner leading to its own degradation (PubMed:19229105). Also polyubiquitinated by RNF41 for proteasomal degradation. S-nitrosylated. The inhibition of PRKN ubiquitin E3 ligase activity by S-nitrosylation could contribute to the degenerative process in PD by impairing the ubiquitination of PRKN substrates. Phosphorylation at Ser-65 by PINK1 contributes to activate PRKN activity. It is however not sufficient and requires binding to phosphorylated ubiquitin as well. |
