ARG58203
anti-Paxillin antibody [PXC-10, aka PAX-14]
anti-Paxillin antibody [PXC-10, aka PAX-14] for Western blot and Human,Mouse,Rat
Overview
| Product Description | Mouse Monoclonal antibody [PXC-10, aka PAX-14] recognizes Paxillin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Chk |
| Tested Application | WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | PXC-10, aka PAX-14 |
| Isotype | IgG1 |
| Target Name | Paxillin |
| Antigen Species | Chicken |
| Immunogen | C-terminal partial recombinant protein corresponding to aa. 305-559 of Chicken Paxillin. |
| Conjugation | Un-conjugated |
| Alternate Names | Paxillin |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Ascites |
| Buffer | PBS, 0.01% Sodium azide and 1% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 1% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PXN |
| Gene Full Name | paxillin |
| Background | This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011] |
| Function | Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). [UniProt] |
| Calculated MW | 61/65/66 kDa (Isoform alpha/beta/gamma) |
| PTM | Phosphorylated by MAPK1/ERK2 (By similarity). Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. Phosphorylation at Ser-244 by CDK5 reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation. Phosphorylation at Tyr-31 and Tyr-118 by PTK6 promote the activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. Phosphorylation at Ser-250 by SLK is required for PXN redistribution and cell motility (PubMed:23128389). [UniProt] |
