ARG40793

anti-Perforin antibody

anti-Perforin antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human,Mouse,Rat

publication_link Publication1

Overview

Product Description Rabbit Polyclonal antibody recognizes Perforin
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Perforin
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 280-555 of Human Perforin (NP_001076585.1).
Conjugation Un-conjugated
Alternate Names FLH2; P1; PFN1; Cytolysin; HPLH2; PFP; Lymphocyte pore-forming protein; Perforin-1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse kidney
Observed Size 68 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18646 Mouse PRF1

GeneID: 50669 Rat PRF1

GeneID: 5551 Human PRF1

Gene Symbol PRF1
Gene Full Name perforin 1 (pore forming protein)
Background Perforin with structural similarities to complement component C9 that is important in immunity. This protein forms membrane pores that allow the release of granzymes and subsequent cytolysis of target cells. Whether pore formation occurs in the plasma membrane of target cells or in an endosomal membrane inside target cells is subject to debate. Mutations in this gene are associated with a variety of human disease including diabetes, multiple sclerosis, lymphomas, autoimmune lymphoproliferative syndrome (ALPS), aplastic anemia, and familial hemophagocytic lymphohistiocytosis type 2 (FHL2), a rare and lethal autosomal recessive disorder of early childhood. [provided by RefSeq, Aug 2017]
Function Perforin plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes. [UniProt]
Cellular Localization Cytoplasmic granule lumen. Secreted. Cell membrane. Endosome lumen. Note=Stored in cytoplasmic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell. Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change. May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes. [UniProt]
Highlight Related products:
Perforin antibodies; Perforin ELISA Kits; Anti-Rabbit IgG secondary antibodies;
Related news:
Examining CTL/NK-mediated cytotoxicity by ELISA
Calculated MW 61 kDa
PTM N-glycosylated. [UniProt]

Specific References

Combination of FAK inhibitor and cytokine-induced killer cell therapy: An alternative therapeutic strategy for patients with triple-negative breast cancer

IHC-P, WB / Human, Mouse

Cheng-Che Wu et al.
Biomedicine & Pharmacotherapy,  (2023)

publication_link

 

hr_line