ARG62591

anti-Perforin antibody [deltaG9]

anti-Perforin antibody [deltaG9] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunohistochemistry,Immunoprecipitation and Human

Immune System antibody

Overview

Product Description Mouse Monoclonal antibody [deltaG9] recognizes Perforin
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC, IHC-P, IP
Host Mouse
Clonality Monoclonal
Clone deltaG9
Isotype IgG2b
Target Name Perforin
Antigen Species Human
Immunogen Purified granules from human YT lymphoma cells
Conjugation Un-conjugated
Alternate Names FLH2; P1; PFN1; Cytolysin; HPLH2; PFP; Lymphocyte pore-forming protein; Perforin-1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS2 - 8 µg/ml
ICC/IF2 - 8 µg/ml
IHCAssay-dependent
IHC-P2 - 8 µg/ml
IP2 - 8 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified
Buffer PBS, 1.5 mM Thimerosal, 1 mg/ml BSA and 50% Glycerol
Preservative 1.5 mM Thimerosal
Stabilizer 1 mg/ml BSA, 50% Glycerol
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5551 Human PRF1

Swiss-port # P14222 Human Perforin-1

Gene Symbol PRF1
Gene Full Name perforin 1 (pore forming protein)
Background Perforin with structural similarities to complement component C9 that is important in immunity. This protein forms membrane pores that allow the release of granzymes and subsequent cytolysis of target cells. Whether pore formation occurs in the plasma membrane of target cells or in an endosomal membrane inside target cells is subject to debate. Mutations in this gene are associated with a variety of human disease including diabetes, multiple sclerosis, lymphomas, autoimmune lymphoproliferative syndrome (ALPS), aplastic anemia, and familial hemophagocytic lymphohistiocytosis type 2 (FHL2), a rare and lethal autosomal recessive disorder of early childhood. [provided by RefSeq, Aug 2017]
Function Perforin plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes. [UniProt]
Highlight Related products:
Perforin antibodies; Perforin ELISA Kits; Anti-Mouse IgG secondary antibodies;
Related news:
Examining CTL/NK-mediated cytotoxicity by ELISA
Research Area Immune System antibody
Calculated MW 61 kDa
PTM N-glycosylated.