ARG54648
anti-Perforin antibody [deltaG9] (PE)
anti-Perforin antibody [deltaG9] (PE) for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections and Human,Bovine
Immune System antibody
Overview
| Product Description | PE-conjugated Mouse Monoclonal antibody [deltaG9] recognizes Perforin |
|---|---|
| Tested Reactivity | Hu, Bov |
| Tested Application | FACS, ICC/IF, IHC-Fr, IHC-P |
| Specificity | This antibody recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | deltaG9 |
| Isotype | IgG2b |
| Target Name | Perforin |
| Antigen Species | Human |
| Immunogen | Purified granules from human YT lymphoma cell line |
| Conjugation | PE |
| Alternate Names | FLH2; P1; PFN1; Cytolysin; HPLH2; PFP; Lymphocyte pore-forming protein; Perforin-1 |
Application Instructions
| Application Suggestion |
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| Application Note | Flow Cytometry: for intracellular staining. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| Preservative | 15 mM Sodium azide |
| Stabilizer | 0.2% (w/v) high-grade protease free BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Keep the antibody in the dark and keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PRF1 |
| Gene Full Name | perforin 1 (pore forming protein) |
| Background | Perforin with structural similarities to complement component C9 that is important in immunity. This protein forms membrane pores that allow the release of granzymes and subsequent cytolysis of target cells. Whether pore formation occurs in the plasma membrane of target cells or in an endosomal membrane inside target cells is subject to debate. Mutations in this gene are associated with a variety of human disease including diabetes, multiple sclerosis, lymphomas, autoimmune lymphoproliferative syndrome (ALPS), aplastic anemia, and familial hemophagocytic lymphohistiocytosis type 2 (FHL2), a rare and lethal autosomal recessive disorder of early childhood. [provided by RefSeq, Aug 2017] |
| Function | Perforin plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes. [UniProt] |
| Highlight | Related products: Perforin antibodies; Perforin ELISA Kits; Anti-Mouse IgG secondary antibodies; Related news: Examining CTL/NK-mediated cytotoxicity by ELISA |
| Research Area | Immune System antibody |
| Calculated MW | 61 kDa |
| PTM | N-glycosylated. |
