ARG63603

anti-Pericentrin 1 / NUP85 antibody

anti-Pericentrin 1 / NUP85 antibody for Immunoprecipitation,Western blot and Human

Controls and Markers antibody

Overview

Product Description Goat Polyclonal antibody recognizes Pericentrin 1 / NUP85
Tested Reactivity Hu
Predict Reactivity Ms, Rat
Tested Application IP, WB
Specificity Please note that, in contrast to other anti-Pericentrin antibodies available, this ab is NOT expected to recognize Kendrin (also known as Pericentrin 2). This may explain why it recognizes a 75KDa band (the expected size according to NP_079120) and not a 220kDa band (as reported for other anti-pericentrin antibodies).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Pericentrin 1 / NUP85
Antigen Species Human
Immunogen C-ARAIIREGSLEGS
Conjugation Un-conjugated
Alternate Names Nup75; FROUNT; Nucleoporin Nup85; Nucleoporin Nup75; Nuclear pore complex protein Nup85; Pericentrin-1; 85 kDa nucleoporin

Application Instructions

Application Suggestion
Tested Application Dilution
IPAssay - dependent
WB1 - 3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 79902 Human NUP85

Swiss-port # Q9BW27 Human Nuclear pore complex protein Nup85

Background Bidirectional transport of macromolecules between the cytoplasm and nucleus occurs through nuclear pore complexes (NPCs) embedded in the nuclear envelope. NPCs are composed of subcomplexes, and NUP85 is part of one such subcomplex, Nup107-160. [provided by RefSeq, Jul 2008]
Research Area Controls and Markers antibody
Calculated MW 75 kDa