ARG52392
anti-Periostin antibody
anti-Periostin antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Chicken
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Periostin |
|---|---|
| Tested Reactivity | Hu, Ms, Chk |
| Predict Reactivity | Bird, Mamm |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Periostin |
| Antigen Species | Mouse |
| Immunogen | Bacterial fusion protein equivalent to a 188-amino acid polypeptide from the C-terminal region of mouse periostin which is comprised of six small alternatively-spliced exons |
| Conjugation | Un-conjugated |
| Alternate Names | OSF2; PN; Osteoblast-specific factor 2; Periostin; OSF-2; PDLPOSTN |
Application Instructions
| Application Suggestion |
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| Application Note | Specific for the ~93 kDa periostin doublet in mouse lung extract. Consistent with the fact that this antibody is made against an alternatively spliced region of periostin, this antibody recognizes only the largest two of the three forms of periostin recognized on Western blots by the Pan periostin antibody (Cat. # 1622-PERI) and also shows a distinctive staining pattern by immunohistochemistry (data not shown). The antibody works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90º C in 10 mM sodium citRate (pH 6.0)/ 0.1 % Tween-20). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | PBS |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | POSTN |
| Gene Full Name | periostin, osteoblast specific factor |
| Background | Periostin is a matricellular protein, i.e. an extracellular matrix protein that interacts both with other ECM proteins and with cell-surface receptors. Like many other matricellular proteins, the function of periostin is important both in embryonic development and in the remodeling of adult tissues in response to pathological insults. Periostin was originally isolated as an osteoblast-specific marker that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading (Kruzynska-Frejtag A. et al., 2004). Periostin has since been demonstrated to be important in heart valve development and myocardial infarction because it promotes collagen fibrogenesis, inhibits differentiation of progenitor cells into cardiomyocytes and is essential in maintaining the biomechanical properties of the adult myocardium (Norris et al., 2008). |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody |
| Calculated MW | 93 kDa |
| PTM | Gamma-carboxylation is controversial. Gamma-carboxyglutamated; gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation; this may be required for calcium binding (PubMed:18450759). According to a more recent report, does not contain vitamin K-dependent gamma-carboxyglutamate residues (PubMed:26273833). |
