ARG59477

anti-Peroxiredoxin 1 antibody

anti-Peroxiredoxin 1 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF,Flow cytometry and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes Peroxiredoxin 1
Tested Reactivity Hu, Ms
Tested Application FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Peroxiredoxin 1
Antigen Species Human
Immunogen Synthetic peptide of Human Peroxiredoxin 1.
Conjugation Un-conjugated
Alternate Names EC 1.11.1.15; TDPX2; PAG; PRX1; MSP23; Natural killer cell-enhancing factor A; Peroxiredoxin-1; PRXI; NKEFA; PAGA; PAGB; Proliferation-associated gene protein; Thioredoxin peroxidase 2; NKEF-A; Thioredoxin-dependent peroxide reductase 2

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:50
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:1000 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18477 Mouse PRDX1

GeneID: 5052 Human PRDX1

Swiss-port # P35700 Mouse Peroxiredoxin-1

Swiss-port # Q06830 Human Peroxiredoxin-1

Gene Symbol PRDX1
Gene Full Name peroxiredoxin 1
Background This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Four transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jan 2011]
Function Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation (By similarity). [UniProt]
Cellular Localization Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. [UniProt]
Calculated MW 22 kDa
PTM Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity. [UniProt]