ARG66175

anti-Peroxiredoxin 1 antibody

anti-Peroxiredoxin 1 antibody for Western blot,ICC/IF and Human,Mouse,Rat

Overview

Product Description Mouse Monoclonal antibody recognizes Peroxiredoxin 1
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, WB
Specificity The antibody detects endogenous Peroxiredoxin 1 protein.
Host Mouse
Clonality Monoclonal
Target Name Peroxiredoxin 1
Antigen Species Human
Immunogen Recombinant Protein of Human Peroxiredoxin 1.
Conjugation Un-conjugated
Alternate Names EC 1.11.1.15; TDPX2; PAG; PRX1; MSP23; Natural killer cell-enhancing factor A; Peroxiredoxin-1; PRXI; NKEFA; PAGA; PAGB; Proliferation-associated gene protein; Thioredoxin peroxidase 2; NKEF-A; Thioredoxin-dependent peroxide reductase 2

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100 - 1:200
WB1:1000 - 1:3000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 117254 Rat PRDX1

GeneID: 18477 Mouse PRDX1

GeneID: 5052 Human PRDX1

Gene Symbol PRDX1
Gene Full Name peroxiredoxin 1
Background This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Four transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jan 2011]
Function Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation (By similarity). [UniProt]
Calculated MW 22 kDa
PTM Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity.