ARG57066

anti-Peroxiredoxin 3 antibody [1F8]

anti-Peroxiredoxin 3 antibody [1F8] for Western blot,ICC/IF and Human

Overview

Product Description Mouse Monoclonal antibody [1F8] recognizes Peroxiredoxin 3
Tested Reactivity Hu
Tested Application ICC/IF, WB
Host Mouse
Clonality Monoclonal
Clone 1F8
Isotype IgG3, kappa
Target Name Peroxiredoxin 3
Antigen Species Human
Immunogen Recombinant fragment around aa. 63-256 of Human Peroxiredoxin3.
Conjugation Un-conjugated
Alternate Names EC 1.11.1.15; Antioxidant protein 1; Prx-III; SP-22; AOP1; AOP-1; MER5; HBC189; PRO1748; Peroxiredoxin-3; Thioredoxin-dependent peroxide reductase, mitochondrial; prx-III; Peroxiredoxin III; Protein MER5 homolog

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 10% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10935 Human PRDX3

Swiss-port # P30048 Human Thioredoxin-dependent peroxide reductase, mitochondrial

Gene Symbol PRDX3
Gene Full Name peroxiredoxin 3
Background This gene encodes a mitochondrial protein with antioxidant function. The protein is similar to the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase, and it can rescue bacterial resistance to alkylhydroperoxide in E. coli that lack the C22 subunit. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologs suggest that these genes consist of a family that is responsible for the regulation of cellular proliferation, differentiation and antioxidant functions. This family member can protect cells from oxidative stress, and it can promote cell survival in prostate cancer. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1, 3, 13 and 22. [provided by RefSeq, Oct 2014]
Function Involved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical-generating system. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol. [UniProt]
Calculated MW 28 kDa
PTM Phosphorylated by LRRK2; phosphorylation reduces perodixase activity.