ARG63086
anti-Phosphotyrosine antibody [P-TYR-01]
anti-Phosphotyrosine antibody [P-TYR-01] for Flow cytometry,ICC/IF,Western blot and All species
Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [P-TYR-01] recognizes Phosphotyrosine |
|---|---|
| Tested Reactivity | All |
| Tested Application | FACS, ICC/IF, WB |
| Specificity | The clone P-Tyr-01 detects tyrosine phosphorylation in activated cells. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | P-TYR-01 |
| Isotype | IgG1 |
| Target Name | Phosphotyrosine |
| Immunogen | Phosphotyrosine conjugated to bovine serum albumin. |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Research Area | Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
|---|
Clone References
Fully automated synthesis of (phospho)peptide arrays in microtiter plate wells provides efficient access to protein tyrosine kinase characterization.
Phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase csk and is involved in regulation of T cell activation.
T cell receptor signalling results in rapid tyrosine phosphorylation of the linker protein LAT present in detergent-resistant membrane microdomains.
Incorporation of leucocyte GPI-anchored proteins and protein tyrosine kinases into lipid-rich membrane domains of COS-7 cells.
