ARG23771

anti-Plexin A1 antibody

anti-Plexin A1 antibody for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes Plexin A1
Tested Reactivity Hu, Ms, Rat
Tested Application ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB
Specificity This antibody detects a ~220 kDa protein corresponding to the apparent molecular mass of Plexin-A1 on SDS-PAGE immunoblots of neonatal rat brain and Cos-7 cells transfected with mouse Plexin-A1. In immunocytochemistry, this antibody co-localizes with anti-Myc in Cos-7 cells transfected with Myc-tagged mouse Plexin-A1.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Plexin A1
Antigen Species Human
Immunogen Synthetic peptide (coupled to carrier protein) around the semaphorin domain (N-terminal half) of Human Plexin A1. This sequence is highly conserved in Rat and Mouse Plexin A1, and has low homology to other Plexin family members.
Conjugation Un-conjugated
Alternate Names Semaphorin receptor NOV; PLEXIN-A1; PLXN1; NOV; Plexin-A1; NOVP

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA1:2000
ICC/IF1:100
IHC-Fr1:100
IHC-P1:100
IP1:50
WB1:1000
Application Note WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature.
IHC-P: Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 220 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.05% Sodium azide, 50% Glycerol and 1 mg/ml BSA.
Preservative 0.05% Sodium azide
Stabilizer 50% Glycerol and 1 mg/ml BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18844 Mouse PLXNA1

GeneID: 5361 Human PLXNA1

Swiss-port # P70206 Mouse Plexin-A1

Swiss-port # Q9UIW2 Human Plexin-A1

Gene Symbol PLXNA1
Gene Full Name plexin A1
Function Coreceptor for SEMA3A, SEMA3C, SEMA3F and SEMA6D. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm (By similarity). [UniProt]
Calculated MW 211 kDa