ARG62963

anti-Proinsulin (C-peptide) antibody [C-PEP-01] (Biotin)

anti-Proinsulin (C-peptide) antibody [C-PEP-01] (Biotin) for IHC-Formalin-fixed paraffin-embedded sections and Human

Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description Biotin-conjugated Mouse Monoclonal antibody [C-PEP-01] recognizes Proinsulin (C-peptide)
Tested Reactivity Hu
Tested Application IHC-P
Specificity The clone C-PEP-01 reacts specifically with C-peptide, a part of the Proinsulin molecule. Proinsulin consists of the three parts: C-peptide and two long strands of amino acids (alpha and beta chains; later become linked together to form the Insulin molecule).
No cross-reactivity with Insulin or other peptide hormones or proteins was observed.
Host Mouse
Clonality Monoclonal
Clone C-PEP-01
Isotype IgG1
Target Name Proinsulin (C-peptide)
Antigen Species Human
Immunogen Human C-peptide conjugated to bovine serum albumin.
Conjugation Biotin
Alternate Names IDDM; IDDM2; IDDM1; ILPR; MODY10; Insulin; IRDN

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P25 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3630 Human INS

Swiss-port # P01308 Human Insulin

Gene Symbol INS
Gene Full Name insulin
Background From every molecule of Proinsulin, one molecule of Insulin plus one molecule of C-peptide are produced. C-peptide is released into the blood stream in equal amounts to Insulin.
Function Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 12 kDa

Clone References

A monoclonal antibody applicable for determination of C-peptide of human proinsulin by RIA.

Hilgert I et al.
Hybridoma.,  (1991)

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