ARG42330

anti-Prominin 1 antibody [293C3] (APC)

anti-Prominin 1 antibody [293C3] (APC) for Flow cytometry and Human

Overview

Product Description APC-conjugated Mouse Monoclonal antibody [293C3] recognizes Prominin 1
Tested Reactivity Hu
Tested Application FACS
Specificity The mouse monoclonal antibody 293C3 recognizes the extracellular epitope 2 on human CD133 (CD133/2), a 120 kDa glycoprotein of prominin family, expressed e.g. on progenitor cells. This antibody is important for identification of human renal progenitors.
Host Mouse
Clonality Monoclonal
Clone 293C3
Isotype IgG2b
Target Name Prominin 1
Antigen Species Human
Immunogen Human Prominin 1.
Conjugation APC
Alternate Names CORD12; CD133; RP41; Antigen AC133; MCDR2; CD antigen CD133; PROML1; AC133; Prominin-like protein 1; Prominin-1; MSTP061; STGD4

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 100 µl of whole blood or 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified
Buffer PBS and 15 mM Sodium azide.
Preservative 15 mM Sodium azide
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8842 Human PROM1

Swiss-port # O43490 Human Prominin-1

Gene Symbol PROM1
Gene Full Name prominin 1
Background This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Function May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439). [UniProt]
Cellular Localization Apical cell membrane; Multi-pass membrane protein. Cell projection, microvillus membrane; Multi-pass membrane protein. Cell projection, cilium, photoreceptor outer segment. Endoplasmic reticulum. Endoplasmic reticulum-Golgi intermediate compartment. Note=Found in extracellular membrane particles in various body fluids such as cerebrospinal fluid, saliva, seminal fluid and urine. [UniProt]
Calculated MW 97 kDa
PTM Isoform 1 and isoform 2 are glycosylated.

Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis. [UniProt]