ARG54138

anti-Prominin 1 antibody

anti-Prominin 1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cancer antibody; Cell Death antibody; Gene Regulation antibody
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Overview

Product Description Mouse Monoclonal antibody recognizes Prominin 1
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-P, WB
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Target Name Prominin 1
Antigen Species Human
Immunogen Purified recombinant human Prominin-1 protein fragments expressed in E.coli.
Conjugation Un-conjugated
Alternate Names CORD12; CD133; RP41; Antigen AC133; MCDR2; CD antigen CD133; PROML1; AC133; Prominin-like protein 1; Prominin-1; MSTP061; STGD4

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:100-1:200
ICC/IF1:100-1:200
IHC-PAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified
Buffer PBS (pH 7.4), 0.02% Sodium azide, 0.1mg/ml BSA and 50% Glycerol
Preservative 0.02% Sodium azide
Stabilizer 0.1mg/ml BSA, 50% Glycerol
Concentration 0.4 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8842 Human PROM1

Swiss-port # O43490 Human Prominin-1

Gene Symbol PROM1
Background Binds cholesterol in cholesterol-containing plasma membrane microdomains.Proposed to play a role in apical plasma membrane organization of epithelial cells.During early retinal development acts as a key regulator of disk morphogenesis.Involved in regulation of MAPK and Akt signaling pathways.In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner.
Cellular Localization Cell projection > cilium > photoreceptor outer segment. Isoform 1: Apical cell membrane; Multi-pass membrane protein By similarity. Cell projection > microvillus membrane; Multi-pass membrane protein.
Highlight Related products:
Prominin1 antibodies; Anti-Mouse IgG secondary antibodies;
Related news:
Stem cell and the regenerative medicine: Ready for the patients
Research Area Cancer antibody; Cell Death antibody; Gene Regulation antibody
Calculated MW 97 kDa
PTM Isoform 1 and isoform 2 are glycosylated.
Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis.

Customer's Feedback

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Review for anti-Prominin 1 antibody

Application:WB

Sample:HCT116

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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Review for anti-Prominin 1 antibody

Application:WB

Sample:HUVEC

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

The protoapigenone analog WYC0209 targets CD133+ cells: A potential adjuvant agent against cancer stem cells in urothelial cancer therapy.

IHC-P / Human

Bi-Wen Yeh et al.
Toxicol Appl Pharmacol.,  (2020)

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