ARG55778

anti-Proteasome 20S LMP2 antibody

anti-Proteasome 20S LMP2 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

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Overview

Product Description

Rabbit Polyclonal antibody recognizes Proteasome 20S LMP2

Tested Reactivity Hu, Ms
Tested Application FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Proteasome 20S LMP2
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 205-239 (C-terminus) of Human Proteasome 20S LMP2.
Conjugation Un-conjugated
Alternate Names beta1i; Proteasome subunit beta type-9; Really interesting new gene 12 protein; Proteasome chain 7; Multicatalytic endopeptidase complex chain 7; Proteasome subunit beta-1i; Macropain chain 7; RING12; EC 3.4.25.1; LMP2; PSMB6i; Low molecular mass protein 2

Application Instructions

Application Suggestion
Tested Application Dilution
FACS
ICC/IF
IHC-P
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A and immunogen peptide.
Buffer PBS and 0.09% (W/V) Sodium azide.
Preservative 0.09% (W/V) Sodium azide.
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16912 Mouse PSMB9

GeneID: 5698 Human PSMB9

Swiss-port # P28065 Human Proteasome subunit beta type-9

Swiss-port # P28076 Mouse Proteasome subunit beta type-9

Gene Symbol PSMB9
Gene Full Name proteasome (prosome, macropain) subunit, beta type, 9
Background The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. [provided by RefSeq, Mar 2010]
Function The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. [UniProt]
Cellular Localization Cytoplasm. Nucleus
Calculated MW 23 kDa
PTM Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.

Customer's Feedback

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Review for anti-Proteasome 20S LMP2 antibody

Application:WB

Sample:A431, HepG2 and MEF

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC