ARG54014

anti-Pyruvate Dehydrogenase E1 alpha subunit antibody

anti-Pyruvate Dehydrogenase E1 alpha subunit antibody for ICC/IF,Western blot and Human,Mouse

Cancer antibody; Metabolism antibody; Signaling Transduction antibody
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Overview

Product Description Mouse Monoclonal antibody recognizes Pyruvate Dehydrogenase E1 alpha subunit
Tested Reactivity Hu, Ms
Tested Application ICC/IF, WB
Host Mouse
Clonality Monoclonal
Isotype IgG1
Target Name Pyruvate Dehydrogenase E1 alpha subunit
Antigen Species Human
Immunogen Purified recombinant human Pyruvate Dehydrogenase protein fragments expressed in E.coli.
Conjugation Un-conjugated
Alternate Names Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; PHE1A; EC 1.2.4.1; PDHE1-A type I; PDHCE1A; PDHAD; PDHA

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified
Buffer 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% Sodium azide and 50% Glycerol
Preservative 0.2% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18597 Mouse PDHA1

GeneID: 5160 Human PDHA1

Swiss-port # P08559 Human Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochond

Swiss-port # P35486 Mouse Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochond

Gene Symbol PDHA1
Gene Full Name pyruvate dehydrogenase (lipoamide) alpha 1
Background The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
Function The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle. [UniProt]
Cellular Localization Mitochondrion matrix
Research Area Cancer antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 43 kDa
PTM Phosphorylation at Ser-232, Ser-293 and Ser-300 by PDK family kinases inactivates the enzyme; for this phosphorylation at a single site is sufficient. Dephosphorylation at all three sites, i.e. at Ser-232, Ser-293 and Ser-300, is required for reactivation.
Acetylation alters the phosphorylation pattern. Deacetylated by SIRT3 (By similarity).

Customer's Feedback

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Review for anti-Pyruvate Dehydrogenase E1 alpha subunit antibody

Application:WB

Sample:HepG2

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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Review for anti-Pyruvate Dehydrogenase E1 alpha subunit antibody

Application:IF/ICC

Sample:HeLa

Fixation Buffer:100% Methanol

Fixation Time:10 min

Fixation Temperature:RT ºC

Permeabilization Buffer:0.1% Triton X-100

Primary Antibody Dilution Factor:1:100

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:FITC