ARG57847

anti-Pyruvate Dehydrogenase E2 antibody

anti-Pyruvate Dehydrogenase E2 antibody for Western blot,ICC/IF and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes Pyruvate Dehydrogenase E2
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Pyruvate Dehydrogenase E2
Antigen Species Human
Immunogen Recombinant protein of Human Pyruvate Dehydrogenase E2.
Conjugation Un-conjugated
Alternate Names Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial; PDC-E2; Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; M2 antigen complex 70 kDa subunit; EC 2.3.1.12; PBC; Pyruvate dehydrogenase complex component E2; DLTA; 70 kDa mitochondrial autoantigen of primary biliary cirrhosis; PDCE2

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:20 - 1:50
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control 22RV-1
Observed Size ~ 72 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1737 Human DLAT

GeneID: 235339 Mouse DLAT

GeneID: 81654 Rat DLAT

Gene Symbol DLAT
Gene Full Name dihydrolipoamide S-acetyltransferase
Background This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.[provided by RefSeq, Oct 2009]
Function The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle. [UniProt]
Cellular Localization Mitochondrion matrix. [UniProt]
Calculated MW 69 kDa
PTM Delipoylated at Lys-132 and Lys-259 by SIRT4, delipoylation decreases the PHD complex activity. [UniProt]