ARG56880

anti-RAD18 antibody

anti-RAD18 antibody for ICC/IF,Western blot and Human

Overview

Product Description

Rabbit Polyclonal antibody recognizes RAD18

Tested Reactivity Hu
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name RAD18
Antigen Species Human
Immunogen Recombinant protein of Human RAD18.
Conjugation Un-conjugated
Alternate Names RING finger protein 73; RNF73; EC 6.3.2.-; E3 ubiquitin-protein ligase RAD18; hRAD18; hHR18; Postreplication repair protein RAD18

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control K562

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 56852 Human RAD18

Swiss-port # Q9NS91 Human E3 ubiquitin-protein ligase RAD18

Gene Symbol RAD18
Gene Full Name RAD18 E3 ubiquitin protein ligase
Background The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq, Jul 2008]
Function E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity. [UniProt]
Calculated MW 56 kDa