ARG43121

anti-RANK antibody

anti-RANK antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes RANK
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name RANK
Antigen Species Human
Immunogen Synthetic peptide corresponding to aa. 235-262 of Human RANK. (YRKKGKALTANLWHWINEACGRLSGDKE)
Conjugation Un-conjugated
Alternate Names ODFR; Osteoclast differentiation factor receptor; OFE; FEO; CD265; PDB2; CD antigen CD265; RANK; Receptor activator of NF-KB; OPTB7; Tumor necrosis factor receptor superfamily member 11A; TRANCER; LOH18CR1; OSTS

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:200 - 1:1000
WB1:500 - 1:2000
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 80 kDa

Properties

Form Liquid
Purification Immunogen affinity purified.
Buffer 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 21934 Mouse TNFRSF11A

GeneID: 8792 Human TNFRSF11A

Swiss-port # O35305 Mouse Tumor necrosis factor receptor superfamily member 11A

Swiss-port # Q9Y6Q6 Human Tumor necrosis factor receptor superfamily member 11A

Gene Symbol TNFRSF11A
Gene Full Name tumor necrosis factor receptor superfamily, member 11a, NFKB activator
Background The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptors can interact with various TRAF family proteins, through which this receptor induces the activation of NF-kappa B and MAPK8/JNK. This receptor and its ligand are important regulators of the interaction between T cells and dendritic cells. This receptor is also an essential mediator for osteoclast and lymph node development. Mutations at this locus have been associated with familial expansile osteolysis, autosomal recessive osteopetrosis, and Paget disease of bone. Alternatively spliced transcript variants have been described for this locus. [provided by RefSeq, Aug 2012]
Function Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells. [UniProt]
Cellular Localization Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform RANK-e5a: Cell membrane; Single-pass type I membrane protein. [UniProt]
Calculated MW 66 kDa