ARG40165

anti-RAP80 antibody

anti-RAP80 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes RAP80
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name RAP80
Antigen Species Human
Immunogen Synthetic peptide derived from Human RAP80.
Conjugation Un-conjugated
Alternate Names RAP80; Retinoid X receptor-interacting protein 110; Receptor-associated protein 80; Ubiquitin interaction motif-containing protein 1; X2HRIP110; BRCA1-A complex subunit RAP80

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:200
WB1:1000 - 1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control 293T
Observed Size ~ 80 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 51720 Human UIMC1

Swiss-port # Q96RL1 Human BRCA1-A complex subunit RAP80

Gene Symbol UIMC1
Gene Full Name ubiquitin interaction motif containing 1
Function Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1. [UniProt]
Cellular Localization Nucleus. Note=Localizes at sites of DNA damage at double-strand breaks (DSBs). [UniProt]
Calculated MW 80 kDa
PTM Sumoylated.

Phosphorylated upon DNA damage by ATM or ATR. [UniProt]