ARG58952

anti-RASA1 antibody

anti-RASA1 antibody for Western blot and Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes RASA1
Tested Reactivity Ms, Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name RASA1
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 140-220 of Human RASA1 (NP_002881.1).
Conjugation Un-conjugated
Alternate Names CM-AVM; PKWS; RASA; RasGAP; Ras p21 protein activator; GAP; p120RASGAP; Ras GTPase-activating protein 1; GTPase-activating protein; p120GAP; CMAVM; RASGAP

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse testis
Observed Size 125 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 25676 Rat RASA1

Swiss-port # P50904 Rat Ras GTPase-activating protein 1

Gene Symbol RASA1
Gene Full Name RAS p21 protein activator (GTPase activating protein) 1
Background The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Mutations also have been associated with hereditary capillary malformations (CM) with or without arteriovenous malformations (AVM) and Parkes Weber syndrome. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues. [provided by RefSeq, May 2012]
Function Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase of normal but not oncogenic Ras p21; this stimulation may be further increased in the presence of NCK1. [UniProt]
Cellular Localization Cytoplasm. [UniProt]
Calculated MW 116 kDa
PTM The N-terminus is blocked.

Phosphorylated by SRC and LCK. The phosphorylation SRC inhibits its ability to stimulate the Ras-GTPase activity, whereas phosphorylation by LCK does not display any effect on stimulation activity. [UniProt]