ARG41302

anti-RBBP6 antibody

anti-RBBP6 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes RBBP6
Tested Reactivity Hu, Ms
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name RBBP6
Antigen Species Human
Immunogen Recombinant protein corresponding to aa. 97-351 of Human RBBP6.
Conjugation Un-conjugated
Alternate Names PACT; Retinoblastoma-binding protein 6; E3 ubiquitin-protein ligase RBBP6; EC 6.3.2.-; SNAMA; Proliferation potential-related protein; RBQ-1; Protein P2P-R; Retinoblastoma-binding Q protein 1; MY038; P2P-R; p53-associated cellular protein of testis

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100
IHC-P1:100
WB2 - 5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse brain

Properties

Form Liquid
Purification Purification with Protein G.
Buffer 0.01M PBS (pH 7.4), 0.03% Proclin 300 and 50% Glycerol.
Preservative 0.03% Proclin 300
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 19647 Mouse RBBP6

GeneID: 5930 Human RBBP6

Swiss-port # P97868 Mouse E3 ubiquitin-protein ligase RBBP6

Swiss-port # Q7Z6E9 Human E3 ubiquitin-protein ligase RBBP6

Gene Symbol RBBP6
Gene Full Name retinoblastoma binding protein 6
Background The retinoblastoma tumor suppressor (pRB) protein binds with many other proteins. In various human cancers, pRB suppresses cellular proliferation and is inactivated. Cell cycle-dependent phosphorylation regulates the activity of pRB. This gene encodes a protein which binds to underphosphorylated but not phosphorylated pRB. Multiple alternatively spliced transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function E3 ubiquitin-protein ligase which promotes ubiquitination of YBX1, leading to its degradation by the proteasome. May play a role as a scaffold protein to promote the assembly of the p53/TP53-MDM2 complex, resulting in increase of MDM2-mediated ubiquitination and degradation of p53/TP53; may function as negative regulator of p53/TP53, leading to both apoptosis and cell growth. [UniProt]
Cellular Localization Nucleus, nucleolus. Chromosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Colocalizes with mitotic chromosomes. Colocalizes with NEK6 in the centrosome. [UniProt]
Calculated MW 202 kDa
PTM Phosphorylated by NEK6. [UniProt]