ARG56228

anti-RBBP7 / RbAp46 antibody

anti-RBBP7 / RbAp46 antibody for Western blot,Flow cytometry and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes RBBP7 / RbAp46
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Bov, Mk, Xenopus
Tested Application FACS, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name RBBP7 / RbAp46
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 1-30 (N-terminus) of Human RBBP7 / RbAp46.
Conjugation Un-conjugated
Alternate Names Retinoblastoma-binding protein 7; Nucleosome-remodeling factor subunit RBAP46; Retinoblastoma-binding protein p46; RBBP-7; RbAp46; Histone acetyltransferase type B subunit 2; Histone-binding protein RBBP7

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:50
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa

Properties

Form Liquid
Purification Purification with Protein A and immunogen peptide.
Buffer PBS and 0.09% (W/V) Sodium azide.
Preservative 0.09% (W/V) Sodium azide.
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5931 Human RBBP7

Swiss-port # Q16576 Human Histone-binding protein RBBP7

Gene Symbol RBBP7
Gene Full Name retinoblastoma binding protein 7
Background This protein is a ubiquitously expressed nuclear protein and belongs to a highly conserved subfamily of WD-repeat proteins. It is found among several proteins that binds directly to retinoblastoma protein, which regulates cell proliferation. The encoded protein is found in many histone deacetylase complexes, including mSin3 co-repressor complex. It is also present in protein complexes involved in chromatin assembly. This protein can interact with BRCA1 tumor-suppressor gene and may have a role in the regulation of cell proliferation and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
Function Core histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the type B histone acetyltransferase (HAT) complex, which is required for chromatin assembly following DNA replication; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; and the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex. [UniProt]
Cellular Localization Nucleus.
Calculated MW 48 kDa