ARG42822
anti-RBL2 / p130 phospho (Thr986) antibody
anti-RBL2 / p130 phospho (Thr986) antibody for Western blot,Flow cytometry,Immunoprecipitation and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes RBL2 / p130 phospho (Thr986) |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | FACS, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RBL2 / p130 |
| Antigen Species | Human |
| Immunogen | Phosphospecific peptide around Thr986 of Human RBL2 / p130. |
| Conjugation | Un-conjugated |
| Alternate Names | RBR-2; p130; Retinoblastoma-related protein 2; Rb2; P130; Retinoblastoma-like protein 2; 130 kDa retinoblastoma-associated protein; pRb2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | K562 | ||||||||
| Observed Size | ~ 130 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | 50 nM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RBL2 |
| Gene Full Name | retinoblastoma-like 2 |
| Function | Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 128 kDa |
| PTM | During G0 and early G1 phase of the cell cycle, phosphorylated on Ser-639 and on 5 sites within the domain B. Phosphorylation on Ser-672 in G1 leads to its ubiquitin-dependent proteolysis. [UniProt] |
