ARG42822

anti-RBL2 / p130 phospho (Thr986) antibody

anti-RBL2 / p130 phospho (Thr986) antibody for Western blot,Flow cytometry,Immunoprecipitation and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes RBL2 / p130 phospho (Thr986)
Tested Reactivity Hu, Ms
Tested Application FACS, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name RBL2 / p130
Antigen Species Human
Immunogen Phosphospecific peptide around Thr986 of Human RBL2 / p130.
Conjugation Un-conjugated
Alternate Names RBR-2; p130; Retinoblastoma-related protein 2; Rb2; P130; Retinoblastoma-like protein 2; 130 kDa retinoblastoma-associated protein; pRb2

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:50
IP1:20
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control K562
Observed Size ~ 130 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer 50 nM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 19651 Mouse RBL2

GeneID: 5934 Human RBL2

Swiss-port # Q08999 Human Retinoblastoma-like protein 2

Swiss-port # Q64700 Mouse Retinoblastoma-like protein 2

Gene Symbol RBL2
Gene Full Name retinoblastoma-like 2
Function Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor. [UniProt]
Cellular Localization Nucleus. [UniProt]
Calculated MW 128 kDa
PTM During G0 and early G1 phase of the cell cycle, phosphorylated on Ser-639 and on 5 sites within the domain B. Phosphorylation on Ser-672 in G1 leads to its ubiquitin-dependent proteolysis. [UniProt]