ARG43162
anti-RBPJK antibody
anti-RBPJK antibody for IHC-Formalin-fixed paraffin-embedded sections and Human,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes RBPJK |
|---|---|
| Tested Reactivity | Hu, Rat |
| Predict Reactivity | Ms |
| Tested Application | IHC-P |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RBPJK |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-260 of Human RBPJK (NP_976028.1). |
| Conjugation | Un-conjugated |
| Alternate Names | CBF1; csl; IGKJRB1; RBPJK; IGKJRB; RBP-J kappa; Recombining binding protein suppressor of hairless; SUH; J kappa-recombination signal-binding protein; AOS3; RBP-J; RBP-JK; KBF2; CBF-1; RBPSUH; Renal carcinoma antigen NY-REN-30 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q06330 Human Recombining binding protein suppressor of hairless |
|---|---|
| Gene Symbol | RBPJ |
| Gene Full Name | recombination signal binding protein for immunoglobulin kappa J region |
| Background | The protein encoded by this gene is a transcriptional regulator important in the Notch signaling pathway. The encoded protein acts as a repressor when not bound to Notch proteins and an activator when bound to Notch proteins. It is thought to function by recruiting chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins to Notch signaling pathway genes. Several transcript variants encoding different isoforms have been found for this gene, and several pseudogenes of this gene exist on chromosome 9. [provided by RefSeq, Oct 2013] |
| Function | Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA (PubMed:21991380). Binds to the oxygen responsive element of COX4I2 and activates its transcription under hypoxia conditions (4% oxygen) (PubMed:23303788). Negatively regulates the phagocyte oxidative burst in response to bacterial infection by repressing transcription of NADPH oxidase subunits (By similarity). [UniProt] |
| Cellular Localization | Nucleus. Cytoplasm. Note=Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm, down-regulating the Notch signaling pathway. [UniProt] |
| Calculated MW | 56 kDa |
