ARG41277

anti-RFC3 antibody

anti-RFC3 antibody for Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes RFC3
Tested Reactivity Hu, Ms, Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name RFC3
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-280 of Human RFC3 (NP_002906.1).
Conjugation Un-conjugated
Alternate Names Activator 1 38 kDa subunit; Activator 1 subunit 3; RFC38; A1 38 kDa subunit; Replication factor C subunit 3; Replication factor C 38 kDa subunit; RF-C 38 kDa subunit

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control 293T
Observed Size 41 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5983 Human RFC3

GeneID: 69263 Mouse RFC3

Swiss-port # P40938 Human Replication factor C subunit 3

Swiss-port # Q8R323 Mouse Replication factor C subunit 3

Gene Symbol RFC3
Gene Full Name replication factor C (activator 1) 3, 38kDa
Background The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
Function The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. [UniProt]
Cellular Localization Nucleus. [UniProt]
Calculated MW 41 kDa